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lambda apc vio770 miltenyi  (Miltenyi Biotec)


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    Miltenyi Biotec lambda apc vio770 miltenyi
    Lambda Apc Vio770 Miltenyi, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ig+lambda+light+chain/Ig+%CE%BB+Light+Chain+Antibody%2C+anti-human/pm41963633-395-80-82
    Average 91 stars, based on 8 article reviews
    lambda apc vio770 miltenyi - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Incubation:

    Article Title: Blocking α 4 β 7 integrin delays viral rebound in SHIV SF162P3 -infected macaques treated with anti-HIV broadly neutralizing antibodies
    Article Snippet: .. Plates were washed and incubated with anti-Ig human lambda light chain–biotin (Miltenyi Biotec), which does not recognize the kappa chain of the Rh-α 4 β 7 for 1 hour at room temperature. .. Plates were washed and incubated with diluted streptavidin-HRP (Invitrogen) for 1 hour at room temperature.

    Article Title: Safety and Immunological Evaluation of Interleukin-21 Plus Anti-α4β7 mAb Combination Therapy in Rhesus Macaques
    Article Snippet: .. After 1-h incubation at RT, plates were washed six times with wash buffer followed by the addition of 100 μL of a 1:100 dilution of a monoclonal anti–lambda light chain-biotin (clone IS7-24C7; Miltenyi Biotech, Cologne, Germany) per well. .. After 1-h incubation at RT, plates were washed six times with wash buffer followed by the addition of 100 μL of a 1:10,000 dilution of streptavidin–horseradish peroxidase (HRP) (Invitrogen) per well.

    Staining:

    Article Title: Vaccine elicitation of HIV broadly neutralizing antibodies from genome-edited B cells in non-human primates and derived lymphoid organoids.
    Article Snippet: .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS. .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS.

    Amplification:

    Article Title: Vaccine elicitation of HIV broadly neutralizing antibodies from genome-edited B cells in non-human primates and derived lymphoid organoids.
    Article Snippet: .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS. .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS.

    Magnetic Cell Separation:

    Article Title: Vaccine elicitation of HIV broadly neutralizing antibodies from genome-edited B cells in non-human primates and derived lymphoid organoids.
    Article Snippet: .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS. .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS.

    FACS:

    Article Title: Vaccine elicitation of HIV broadly neutralizing antibodies from genome-edited B cells in non-human primates and derived lymphoid organoids.
    Article Snippet: .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS. .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS.

    other:

    Article Title: Preclinical safety and efficacy of a therapeutic antibody that targets SARS-CoV-2 at the sotrovimab face but is escaped by Omicron
    Article Snippet: Anti-Lambda-PE (clone IS7-24C) , Miltenyi Biotec , Cat# 130-119-778; RRID: AB_2751837.




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    Image Search Results


    Journal: iScience

    Article Title: Preclinical safety and efficacy of a therapeutic antibody that targets SARS-CoV-2 at the sotrovimab face but is escaped by Omicron

    doi: 10.1016/j.isci.2023.106323

    Figure Lengend Snippet:

    Article Snippet: Anti-Lambda-PE (clone IS7-24C) , Miltenyi Biotec , Cat# 130-119-778; RRID: AB_2751837.

    Techniques: Virus, Recombinant, Blocking Assay, Saline, Enzyme-linked Immunosorbent Assay, Membrane, Plasmid Preparation, Expressing, Software

    B cells were enriched from blood of 3 healthy volunteers and infected with live or UV-inactivated purified PUUV, together with Sendai and Sindbis viruses, for comparison (multiplicity of infections for all viruses equals 1). The TLR9 agonist CpG and no treatment were used as a positive and negative controls for B cell activation, respectively. After culturing for 8 days, kappa and lambda FLCs in (A) and IgA, IgM and IgGs in (B) were measured from cell supernatants. Remaining cells were subjected to IgA, IgM and IgG-specific antibody secreting cell (ASC) quantification using Elispot. IgA + and IgM + ASCs were simultaneously detected in one well, using AP-conjugated anti-IgA and HRP-conjugated anti-IgM Abs, and IgG + ASCs in a separate well using HRP-conjugated anti-IgG Ab. Representative Elispot images from one donor are shown in (C), and ASC quantification per input cell number measured from all donors in (D). Significant differences of each treatment group, as compared to non-treated cells, were assessed using two-way ANOVA and Dunnett’s multiple comparison tests. *p < 0.05, **p < 0.01, ***p < 0.001.

    Journal: PLoS Pathogens

    Article Title: Hantavirus infection-induced B cell activation elevates free light chains levels in circulation

    doi: 10.1371/journal.ppat.1009843

    Figure Lengend Snippet: B cells were enriched from blood of 3 healthy volunteers and infected with live or UV-inactivated purified PUUV, together with Sendai and Sindbis viruses, for comparison (multiplicity of infections for all viruses equals 1). The TLR9 agonist CpG and no treatment were used as a positive and negative controls for B cell activation, respectively. After culturing for 8 days, kappa and lambda FLCs in (A) and IgA, IgM and IgGs in (B) were measured from cell supernatants. Remaining cells were subjected to IgA, IgM and IgG-specific antibody secreting cell (ASC) quantification using Elispot. IgA + and IgM + ASCs were simultaneously detected in one well, using AP-conjugated anti-IgA and HRP-conjugated anti-IgM Abs, and IgG + ASCs in a separate well using HRP-conjugated anti-IgG Ab. Representative Elispot images from one donor are shown in (C), and ASC quantification per input cell number measured from all donors in (D). Significant differences of each treatment group, as compared to non-treated cells, were assessed using two-way ANOVA and Dunnett’s multiple comparison tests. *p < 0.05, **p < 0.01, ***p < 0.001.

    Article Snippet: The following mAbs were used: anti-kappa light chain PercP-Cy5.5 (G20-193), anti-lambda light chain BV605 (JDC-12), anti-IgM APC (G20-127), anti-IgG AF700 (G18-145; all from BD) and anti-IgA PE-Vio770 (IS11-8E10; Miltenyi).

    Techniques: Infection, Purification, Comparison, Activation Assay, Enzyme-linked Immunospot